Summary

PATCH CLAMP in vitro

 

Cellular Models

In vitro cellular models are powerful tools for the study of brain disorders. The cellular models commonly used in the core facility are:

  • iPSC-derived neurons
  • Primary cell culture
  • Cell lines expressing the ion channel of interest

human IPSC-derived neurons

Primary neuronal culture

Readouts

  • Ion channel biophysical properties
  • Current density
  • Intrinsic membrane properties
  • Action potential firing and excitability
  • Spontaneous post-synaptic potential/current

Applications

  • Functional screening of channelopathies
  • Characterization of the maturation of neuronal culture (spiking profile and synaptic connectivity)
  •  Phenotyping of preclinical model of diseases
  •  Target valdiation for drug dsicovery
  •  Exploration of the effect of drug on membrane excitablity

Highlights

Functional screening of channelopathies

Fonctional caracterisation of HCN1 mutation from epileptic encephalopathy to benign generalized epilepsy and beyond. Publication

Characterization of the maturation of neuronal culture

Fonctional characterization of the human IPSC-derived neurons in Parkinson’s disease. Publication

Services

  • Generation of full data set : experiments design, data acquisition and analysis
  • Access to the facility setup for trained electrophysiologists
  • Training in electrophysiology
  • R&D assays
  • Consulting

ex vivo PATCH-CLAMP

MULTIELECTRODE ARRAY


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