in vitro, ex vivo MULTIELECTRODE ARRAY

The multielectrode array (MEA) system is an user-friendly station for in vitro and ex vivo extracellular electrophysiology. Brain slice or primary cultured cells are placed or grown directly on a grid of planar microelectrodes. Each electrode simultaneously records field potential. Stimulation can be deliver via any electrode. MEA technology allows to assess important physiological functions of the brain as synaptic plasticity and network activity.

Equipment

64 electrodes	Probe	Chamber

• 64 electrodes for simultaneous extracellular recordings
• Stimulation through 1 or 2 electrodes  sequentially
• Suitable for cell cultures, brain organoids and brain slices
• Upgrade of the MEA system with multiwells probes

The core facility uses the MED64 system from Alpha Med Scientific Ltd.

Readout: Spike Firing

64 channels dispaying electrical activity Recorded from 64 electrodes (neuronal cortical culturE)

• Spike frequency
• Synchronization
• Oscillations

Experimental Models: primary cell cultures, brain organoids and brain slices

Readout: Synaptic Activity

• Field evoked postsynaptic potentials (PSPs)
• Short-term plasticity
• Long-term potentiation (LTP) and depression (LTD)  of synaptic transmission

Experimental Models: brain slices

Assays

• Characterization stem cell differentiations for mature phenotype
• Synaptic plasticity
• Characterization of neuronal excitability
• Functional activity of neuronal cultures
• Pharmacological study on neurotransmission

Services

• Generation of full data set : experiments design, data acquisition and analysis
• Access to the facility setup for trained electrophysiologists
• Training in electrophysiology
• R&D assays
• Consulting